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368 DIFFERENTIAL SUPPRESSION OF DENGUE VIRUS REPLICATION BY NITRIC OXIDE. Ubol S, Takumpanya R, Mammen PM, Endy PT, Libraty D. Department of Microbiology, Mahidol University, Bangkok, Thailand; Division of Virology, Armed Force Research Institute of Medical Science, Bangkok, Thailand; Department of Medicine and Infectious Diseases, CIDVR, University of Massachusetts School of Medicine. Dengue virus DV ; is a member of an arthropod-born Flavivirus which affects up to 100 million individuals annually. It causes a spectrum of diseases ranging from asymptomatic infection, mild illness to severe disease with fatal. DV actively replicates in various types of immune cells especially the one that is well accepted as professional nitric oxide producer, monocyte macrophage and dendritic cell. The interaction between nitric oxide NO ; and DV in these target cells is unknown. Therefore, we performed an experiment to investigate the effect of NO on vitro replication of 26 isolates of DV. These viruses are primarily isolates from DF and DHF patients 13 DF and 13 DHF ; . N18 cells were infected with either DF or DHF isolate and immediately treated with 50 or 75 SNAP, an exogenous NO donor. Production of viral progenies in the present and absent of NO was followed by monitoring the copy number of genomic viral RNA using TaqMan RT-PCR. It was found that NO exerted an inhibitory effect on DV replication. SNAP at 75 M generated NO high enough to suppress all 26 isolates. However, at lower concentration of NO, 50uM SNAP, the susceptibility of DF and DHF isolates to inhibitory effect of NO was difference. We found that 77% of DF isolates were susceptible while only 54% of DHF isolates were affected. In conclusion, NO negatively regulates DV replication. The degree of suppression is dependent on phenotype of virus and viagra.
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SEARCHING FOR A MARKER OF REM PROPENSITY IN HUMANS Wong R, 3 Wetherell L, 1, 3 Schlosser A, 4, 3 Orff HJ, 5, 2 Drummond S1, 2 1 ; Department of Psychiatry, University of California, San Diego, San Diego, CA, USA, 2 ; Psychology Service, Veterans Affairs San Diego Healthcare System, San Diego, CA, USA, 3 ; Research Service, Veterans Affairs San Diego Healthcare System, San Diego, CA, USA, 4 ; General Clinical Research Center, University of California, San Diego, San Diego, CA, USA, 5 ; Joint Doctoral Program in Clinical Psychology, San Diego State University University of California, San Diego, San Diego, CA, USA Introduction : Delta activity during sleep and Theta activity while awake increase proportionately with the homeostatic drive for sleep, and are understood to be markers for sleep propensity. There is no accepted EEG marker for REM propensity in humans, although REM Theta has been correlated with REM density REMd ; in animals. Alpha is thought to be negatively correlated with REM measures. Here we examined whether Power Spectral Analysis PSA ; of the EEG during REM could clarify whether Theta can serve as a marker of REM propensity or intensity. Methods : 30 subjects age 24.34.7, 15F ; participated in a 6 consecutive night study in the sleep lab: Screen SCR ; , Baseline BL ; , TSD DEP1 & DEP2 ; , and Recovery REC1 & REC2 ; . PSA and hierarchical regression were used to examine the relationship between relative power in three frequency bins [Theta 4-7.5Hz ; , Delta .5-4Hz ; , and Alpha 7.510Hz ; ] during REM and two measures of REM propensity REM% and REMd ; . Results : Theta accounted for a significant amount of the variance in REM% during BL, REC1 and REC2 p .002 ; . While Alpha contributed to variance explained during REC1 p .01 ; , Delta did not add to variance accounted for on any night. Spectral power in all 3 bands was much less related to REMd, with the only significant relationships being Theta for and zanaflex.
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Doubtedly it would be necessary to demonstrate that STM can be suppressed without affecting LTM of the same learning experience in the same animal, or that this is utterly impossible Izquierdo 1989, Izquierdo et al. 1998a, 1999 ; . Here we review the effect on STM and LTM of drugs known to affect specific steps of LTM formation when given into CA1 region of rats hippocampal area Ardenghi et al. 1997, Bernabeu et al. 1997a, b, Bevilaqua et al. 1997, Cammarota et al. 1997, Izquierdo & Medina 1998, Izquierdo & Medina 1995 ; . The drugs used exert specific effects upon neurotransmitter receptors or enzymes activities crucial to plastic processes Bernabeu et al. 1997a, Cammarota et al. 1998, Izquierdo & Medina 1997, Izquierdo & Medina 1995 ; . Memory Types Memories are classified according to their content declarative or explicit, procedural or implicit ; Squire 1992, Markowitsch 1997 ; , according to their duration STM, LTM ; Fuster 1998, Markowitsch 1997 ; , and according to their nature: archival STM, LTM ; as opposed to transient, moment-to-moment WM ; Goldman-Rakic 1992, 1996 ; . The present article deals with STM and LTM of one-trial inhibitory or passive avoidance Gold 1986, Izquierdo & Medina 1997 ; . This might be viewed as an explicit memory, to the point that terms such as "declarative" or "explicit" can be applied to experiments using rodents Bures 1998 ; . Putative subdivisions of STM and LTM see Markowitsch 1997 and Squire 1992 ; will be ignored, inasmuch as they are irrelevant to the findings discussed here, and there is no tangible biological basis to substantiate any such subdivision Izquierdo et al. 1999 ; . Here, therefore, we will restrict ourselves to McGaugh's 1968 ; concept of "three memory trace systems: one for immediate memory, one for short-term memory which develops in a few seconds or minutes and lasts for several hours ; , and one which consolidates slowly and is relatively permanent" see also McGaugh 2000.
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